Instrument setup


Matrix-assisted laser desorption/ionization time of flight mass spectrometry

MALDI/TOF-MS analysis of permethylated glycosphingolipids is performed in the linear or reflector positive ion mode using α-dihyroxybenzoic acid (DHBA, 20mg/mL solution in 50%methanol: water) as a matrix.


Nanospray ionization mass spectrometry

Mass analysis by NSI-MS are performed by direct infusion of permethylated glycosphingolipids dissolved in infusion buffer the instrument at a constant flow rate of 0.4~1 μL/min via a syringe pump.